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1986•Journal of BacteriologyOpen access

Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria

Georg Schmetterer, C. Peter Wölk, Jeff Elhai

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Abstract

Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.

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What this paper is about

Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.

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Available abstract

Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.

Key concepts: Vibrio harveyi, Biology, Vibrio, Luciferases, Microbiology, Heterocyst, Shuttle vector, Cyanobacteria

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Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria — Research Paper | ScholarLens