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U937 cell line has a t(I0;II)(pI3–I4;qI4–2I) rather than a deletion of IIq

Hirofumi Kobayashi, Michael J. Thirman, Janet D. Rowley

Open publisher page 10 citations

Abstract

The U937 cell line was studied with the fluorescence in situ hybridization (FISH) technique using phage and cosmid probes which were mapped and ordered on 11q. Although this cell line was thought to have a del(11q), FISH demonstrated that 11q was translocated to 10p and that the breakpoint on 11q is centromeric to the MLL gene. This 10;11 translocation appears to be a t(10;11)(p13-14;q14-21), which was recently reported to be a recurring translocation in malignant hematologic disease. This cell line will be a good tool for the study of this chromosomal rearrangement.

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What this paper is about

The U937 cell line was studied with the fluorescence in situ hybridization (FISH) technique using phage and cosmid probes which were mapped and ordered on 11q. Although this cell line was thought to have a del(11q), FISH demonstrated that 11q was translocated to 10p and that the breakpoint on 11q is centromeric to the MLL gene. This 10;11 translocation appears to be a t(10;11)(p13-14;q14-21), which was recently reported to be a recurring translocation in malignant hematologic disease. This cell line will be a good tool for the study of this chromosomal rearrangement.

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OpenAlex reports 10 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The U937 cell line was studied with the fluorescence in situ hybridization (FISH) technique using phage and cosmid probes which were mapped and ordered on 11q. Although this cell line was thought to have a del(11q), FISH demonstrated that 11q was translocated to 10p and that the breakpoint on 11q is centromeric to the MLL gene. This 10;11 translocation appears to be a t(10;11)(p13-14;q14-21), which was recently reported to be a recurring translocation in malignant hematologic disease. This cell line will be a good tool for the study of this chromosomal rearrangement.

Key concepts: Cosmid, Chromosomal translocation, Fluorescence in situ hybridization, Breakpoint, Fish <Actinopterygii>, Cell culture, Biology, Molecular biology

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U937 cell line has a t(I0;II)(pI3–I4;qI4–2I) rather than a deletion of IIq — Research Paper | ScholarLens