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1935•The Journal of Comparative NeurologyRequires access

Histological analysis of the Nissl‐pattern and ‐substance of nerve cells

Lárus Einarson

Open publisher page 37 citations

Abstract

Abstract The influence of the variety and pH of the fixative upon the Nissl pattern has been studied, together with the action of warm water, acids and Na2CO3 after ordinary fixation. The effect of NH4OH is characteristic. The stainability is considerably modified, but through alternating the use of the gallocyanin method and of a new stain, gallamin blue, desribed in the text, the Nissl substance is always stainable. After fixation in alcohol the substance does not stain with purified methylgreen.

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Abstract The influence of the variety and pH of the fixative upon the Nissl pattern has been studied, together with the action of warm water, acids and Na2CO3 after ordinary fixation. The effect of NH4OH is characteristic. The stainability is considerably modified, but through alternating the use of the gallocyanin method and of a new stain, gallamin blue, desribed in the text, the Nissl substance is always stainable. After fixation in alcohol the substance does not stain with purified methylgreen.

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Available abstract

Abstract The influence of the variety and pH of the fixative upon the Nissl pattern has been studied, together with the action of warm water, acids and Na2CO3 after ordinary fixation. The effect of NH4OH is characteristic. The stainability is considerably modified, but through alternating the use of the gallocyanin method and of a new stain, gallamin blue, desribed in the text, the Nissl substance is always stainable. After fixation in alcohol the substance does not stain with purified methylgreen.

Key concepts: Nissl body, Fixative, Stain, Biology, Fixation (population genetics), Substance P, Nerve cells, Staining

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