2011•Journal of Clinical MicrobiologyOpen access

Multiplex Real-Time PCR Assay and Melting Curve Analysis for Identifying Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria

Jeong-Uk Kim, Choong‐Hwan Cha, Hae-Kyong An

Abstract

A multiplex real-time PCR assay and melting curve analysis for identifying 23 mycobacterial species was developed and evaluated using 77 reference strains and 369 clinical isolates. Concordant results were obtained for all 189 (100%) isolates of the Mycobacterium tuberculosis complex and 169 (93.9%) isolates of nontuberculous mycobacteria. Our results showed that this multiplex real-time PCR assay is an effective tool for the mycobacterial identification from cultures.

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Key concepts: Nontuberculous mycobacteria, Melting curve analysis, Multiplex, Mycobacterium tuberculosis complex, Mycobacterium tuberculosis, Multiplex polymerase chain reaction, Mycobacterium, Microbiology