Evaluation of hydrolytic enzyme activities of endophytes from some indigenous medicinal plants.

The evaluation of some endophytes was carried out for their possible hydrolytic enzymatic activity from various parts of medicinal plants belonging to Jalgaon Maharashtra, India. A total of fourteen bacterial endophytes and twenty four fungal endophytes were isolated from the aerial and underground parts of seven medicinal plants. Six bacterial isolates and fifteen fungal isolates expressed the amylolytic activity. Cellulolytic activity was observed in seven bacterial isolates and three fungal isolates. Nine bacteria and ten fungal isolates were able to produce esterase and lipase enzymes. Amylase, cellulase, esterase and lipase activity occurred in low amount in the endophytic bacteria coded HB1 of rhizomes of Curcuma longa L. in comparison with other endophyte isolates code NB1, Lactobacillus fermentum and Escherichia coli. Proteolytic activity was found in nine bacterial and twelve fungal endophytes, highest potential to hydrolyse of casein and gelatine that was recorded in bacterial isolate coded SB1 from leaves of Vinca rosea (L.) G. Don. Asparaginase activity was noted only by fungal isolates coded NF1 andNF2 and bacterial isolates, coded NMB2 from stem of Azadirachta indica A. Juss.