2005•Encyclopedia of Life SciencesRequires access
Western Blotting Analysis of Translation Products
David B Whitehouse, J. U. Lovegrove
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Abstract
Abstract Western blotting (immunoblotting) is a system for the immunological detection of specific proteins that have been transferred from a polyacrylamide gel to an immobilizing membrane. Proteins transferred to immobilizing filter membranes, such as nitrocellulose, are well suited to high‐sensitivity solid‐phase immunoassay detection techniques.