1993•FEMS Microbiology LettersOpen access

The operon for the Fe-hydrogenase inDesulfovibrio vulgaris(Hildenborough): Mapping of the transcript and regulation of expression

W. van den Berg, Jack P. W. G. Stokkermans, Walter M. A. M. van Dongen

Abstract

The genes for the subunits of the Fe-only hydrogenase from Desulfovibrio vulgaris are transcribed as a 1.9 kb mRNA; the operon contains no other genes besides those encoding the two subunits. The transcriptional start site of the operon was mapped. Determination of hydrogenase activity and hydrogenase mRNA levels indicates a growth-phase dependent regulation of hydrogenase expression at transcriptional level. However, it has not yet been possible to localize the sequences required for regulation and expression of the genes.

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Key concepts: Operon, Hydrogenase, Desulfovibrio vulgaris, Gene, Biology, Transcriptional regulation, Transcription (linguistics), Messenger RNA