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Synergistic Effect of Interferon-γ and Tumor Necrosis Factor-α on Antiviral Activity and (2′–5′) Oligo (A) Synthetase Induction in a Myelomonocytic Cell Line

Juana Wietzerbin, C. Gaudelet, LILIANE CATINOT, Judith Chebath, RÉBÉCA FALCOFF

Open publisher page 19 citations

Abstract

TNF was not observed to have an antiviral effect on either HL60 or U937 cells. However, it did significantly enhance interferon (IFN)-gamma-mediated antiviral activity in U937 cells. Treatment of U937 cells with IFN-gamma enhanced (2'-5') oligo (A) synthetase activity (2.5-fold) but treatment with TNF did not. Combined treatment with TNF-alpha + IFN-gamma increased this activity dramatically (20-40-fold). This increase correlated with the very large increase in the (2'-5') oligo (A) synthetase mRNA level in U937 cells. No such effects were observed in HL60 cells. Furthermore, binding studies and cross-linking analysis showed that IFN-gamma modulated TNF-alpha receptors in U937 cells without altering their properties, but did not do so in HL60 cells.

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What this paper is about

TNF was not observed to have an antiviral effect on either HL60 or U937 cells. However, it did significantly enhance interferon (IFN)-gamma-mediated antiviral activity in U937 cells. Treatment of U937 cells with IFN-gamma enhanced (2'-5') oligo (A) synthetase activity (2.5-fold) but treatment with TNF did not. Combined treatment with TNF-alpha + IFN-gamma increased this activity dramatically (20-40-fold). This increase correlated with the very large increase in the (2'-5') oligo (A) synthetase mRNA level in U937 cells. No such effects were observed in HL60 cells. Furthermore, binding studies and cross-linking analysis showed that IFN-gamma modulated TNF-alpha receptors in U937 cells without altering their properties, but did not do so in HL60 cells.

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Available abstract

TNF was not observed to have an antiviral effect on either HL60 or U937 cells. However, it did significantly enhance interferon (IFN)-gamma-mediated antiviral activity in U937 cells. Treatment of U937 cells with IFN-gamma enhanced (2'-5') oligo (A) synthetase activity (2.5-fold) but treatment with TNF did not. Combined treatment with TNF-alpha + IFN-gamma increased this activity dramatically (20-40-fold). This increase correlated with the very large increase in the (2'-5') oligo (A) synthetase mRNA level in U937 cells. No such effects were observed in HL60 cells. Furthermore, binding studies and cross-linking analysis showed that IFN-gamma modulated TNF-alpha receptors in U937 cells without altering their properties, but did not do so in HL60 cells.

Key concepts: U937 cell, HL60, Biology, Tumor necrosis factor alpha, Interferon, Cell culture, Cytokine, Receptor

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Synergistic Effect of Interferon-γ and Tumor Necrosis Factor-α on Antiviral Activity and (2′–5′) Oligo (A) Synthetase Induction in a Myelomonocytic Cell Line — Research Paper | ScholarLens