1993•Molecular MicrobiologyRequires access

Identification of an operon involved in the assimilatory nitrate‐reducing system of Azotobacter vineiandii

F. Ramos, Gonzalo Blanco, J.C. Gutiérrez, Francisco Luque, Marı́a Tortolero

Abstract

A number of mutants lacking nitrate reductase (Nas-) or nitrite reductase (Nis-) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned from an Azotobacter vinelandii gene bank. nasA encodes for nitrite reductase apoenzyme, whereas nasB is specific for nitrate reductase activity. Nitrate reductase exerts a regulatory effect on nasAB.

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Key concepts: Nitrate reductase, Operon, Azotobacter vinelandii, Nitrite reductase, Biology, Nitrate, Azotobacteraceae, Azotobacter