Induction of protonemal gemmae and gametophyte of Cratoneuron decipien (Brid.) G. Roth using IAA and kinetin

The protonemal gemmae and gametophyte inducing effect of the IAA and kinetin was examined in moss Cratoneuron decipiens growing using solid media. Cultures were raised from gametophyte tips on a solid basal medium containing Knop’s major salts and Nitsch and Nitsch’s trace elements. Aseptic chopped gametophytes were inoculated onto four types of concentrations (10 -8 , 10 -7 , 10 -6 and 10 -5 M) of IAA and kinetin. High frequency of bud and gemmae were selected then subcultured to same supplemented of IAA and kinetin. Protonema produced gemmae, callus and gametophytic bud. Production of protonemal gemmae varies according to the concentration of IAA and kinetin, whereas low concentrations of growth regulators promoted gemmae formation and bud induction. Gametophytes were regenerated from protonema and calli after subculture. IAA regulated gametophytes induction and growth. Kinetin influenced gemmae formation and gametophyte regeneration also. All aspects of development of this moss species are governed by the external growth regulators.