2004•Journal of Clinical MicrobiologyOpen access

Development and Evaluation of Fluorogenic TaqMan Reverse Transcriptase PCR Assays for Detection of Dengue Virus Types 1 to 4

Mikako Ito, Tomohiko Takasaki, Kenichiro Yamada, Reiko Nerome, Shigeru Tajima, Ichiro Kurane

Abstract

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

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Key concepts: TaqMan, Dengue virus, Virology, Reverse transcriptase, Dengue fever, Biology, Taq polymerase, Virus