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2011•Acta Botanica HungaricaRequires access

In vitro micropropagation ofCatharanthus roseus— An anticancer medicinal plant

Abdul Bakrudeen, G. Subha Shanthi, T. Gouthaman, M. Kavitha, M. V. Rao

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Abstract

An efficient protocol was standardized using axillary bud and shoot tip explants of Catharanthus roseus — an anticancer medicinal plant. The highest number of shoots (19.6 shoots / auxiliary node) was observed after 45 days of culture in the MS medium supplemented with NAA (4.0 mg l−1) + BA (4.0 mg l−1). Shoots were proliferated and elongated in the same medium. High frequency of rooting (82.5%) was obtained in half strength MS + IBA (4.0) from axillary bud derived shoots. The rooted plantlets were successfully established in soil.

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What this paper is about

An efficient protocol was standardized using axillary bud and shoot tip explants of Catharanthus roseus — an anticancer medicinal plant. The highest number of shoots (19.6 shoots / auxiliary node) was observed after 45 days of culture in the MS medium supplemented with NAA (4.0 mg l−1) + BA (4.0 mg l−1). Shoots were proliferated and elongated in the same medium. High frequency of rooting (82.5%) was obtained in half strength MS + IBA (4.0) from axillary bud derived shoots. The rooted plantlets were successfully established in soil.

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OpenAlex reports 15 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

An efficient protocol was standardized using axillary bud and shoot tip explants of Catharanthus roseus — an anticancer medicinal plant. The highest number of shoots (19.6 shoots / auxiliary node) was observed after 45 days of culture in the MS medium supplemented with NAA (4.0 mg l−1) + BA (4.0 mg l−1). Shoots were proliferated and elongated in the same medium. High frequency of rooting (82.5%) was obtained in half strength MS + IBA (4.0) from axillary bud derived shoots. The rooted plantlets were successfully established in soil.

Key concepts: Catharanthus roseus, Shoot, Axillary bud, Micropropagation, Explant culture, Biology, Botany, Murashige and Skoog medium

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