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2017•Journal of Microbiology and Biotechnology ResearchOpen access

In Vitro Culture of Jatropha Curcas L. – An Important Medicinal Plant

P. Jeevan, A. Edith Rena, S. Siva Subramanian, R. Nelson

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Abstract

An in vitro protocol for micropropagation of Jatropha curcas L. was developled through nodal explants. Murashige and Skoog (MS) medium with BAP (0.5 mg/L) and Kn (1.0 mg/L) was found to be the best for multiple shoot induction from nodal explants. The maximum response of shoot induction was observed on a medium containing 1.0 mg/L Kn. The regenerated shoots were excised and transferred to rooting medium containing IAA and IBA at different concentrations. Highest of 90 % and 84 % rooting was observed at 1.0 mg/L IAA and 0.5 mg/L IBA respectively. The in vitro derived plantlets were hardened and acclimatized in soils. About 87 % of plantlets survived in the field condition. Keywords: Jatropha curcas, nodal segments, in vitro, acclimatization, IAA.

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An in vitro protocol for micropropagation of Jatropha curcas L. was developled through nodal explants. Murashige and Skoog (MS) medium with BAP (0.5 mg/L) and Kn (1.0 mg/L) was found to be the best for multiple shoot induction from nodal explants. The maximum response of shoot induction was observed on a medium containing 1.0 mg/L Kn. The regenerated shoots were excised and transferred to rooting medium containing IAA and IBA at different concentrations. Highest of 90 % and 84 % rooting was observed at 1.0 mg/L IAA and 0.5 mg/L IBA respectively. The in vitro derived plantlets were hardened and acclimatized in soils. About 87 % of plantlets survived in the field condition. Keywords: Jatropha curcas, nodal segments, in vitro, acclimatization, IAA.

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Available abstract

An in vitro protocol for micropropagation of Jatropha curcas L. was developled through nodal explants. Murashige and Skoog (MS) medium with BAP (0.5 mg/L) and Kn (1.0 mg/L) was found to be the best for multiple shoot induction from nodal explants. The maximum response of shoot induction was observed on a medium containing 1.0 mg/L Kn. The regenerated shoots were excised and transferred to rooting medium containing IAA and IBA at different concentrations. Highest of 90 % and 84 % rooting was observed at 1.0 mg/L IAA and 0.5 mg/L IBA respectively. The in vitro derived plantlets were hardened and acclimatized in soils. About 87 % of plantlets survived in the field condition. Keywords: Jatropha curcas, nodal segments, in vitro, acclimatization, IAA.

Key concepts: Jatropha curcas, Explant culture, Shoot, Acclimatization, Micropropagation, Murashige and Skoog medium, Biology, In vitro

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