1999•Unpublished venueRequires access
Polyploid Induction in Centella asiatica (L.) Urban by Colchicine Treatment
Warawut Chulalaksananukul, Wisa Chimnoi
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Abstract
polyploid induction procedure using 1 and 2 % colchicine. Two methods of application were used, one by placing seedlings on colchicine-saturated cotton balls, the other by placing colchicine-containing agar on seedling shoot tips. Results showed that the cotton ball method with 2 % colchicine gave the highest rate of polyploid induction, while no significant difference was obtained by the agar method. Polyploid plants differed from the diploid ones in the observable phenotypes and containing a higher number of guard cell chloroplasts.