The genome of the olive fruit flyBactrocera oleae: localization of molecular markers by in situ hybridization to the salivary gland polytene chromosomes

Nine specific DNA probes (genomic or cDNA) from Ceratitis capitata have been mapped by in situ hybridization to the salivary gland polytene chromosomes of the olive fruit fly Bactrocera oleae, a major agricultural pest, thus establishing molecular markers for the 5 autosomal chromosomes. Taking into account the present results, as well as previous data obtained mainly by in situ hybridizations, chromosomal homologies among B. oleae, C. capitata and B. tryoni are established. Data show extensive linkage group conservation among the 3 taxa of the economically important and globally distributed family, the Tephritidae.Key words: Bactrocera oleae, Tephritidae, salivary gland, polytene chromosomes, in situ hybridization, mapping.