2009•Applied and Environmental MicrobiologyOpen access

Genetic Analysis for the Lack of Expression of the O157 Antigen in an O Rough:H7 Escherichia coli Strain

Lydia V. Rump, Peter Feng, Markus J. Fischer, Steven R. Monday

Abstract

The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6.

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Key concepts: Escherichia coli, Complementation, Operon, Biology, Strain (injury), Gene, Antigen, Enterobacteriaceae