Photometrical and dosimetrical measurements for photodynamic diagnosis and therapy with ALA and h-ALA induced PpIX

The quantification of the fluorescence is important to design optical equipment for Photodynamic Diagnosis. The fluorescence intensity of Protoporphyrin IX in solution and induced in cells, cultivated in the hen's eggs model, have been measured photometrically. With an optimized CCD-camera-systems fluorescent tumor areas were detected before and after PDT. The ratio of dead cells was detected by staining with trypan blue after PDT. The measurements were carried out various energy densities at the time of maximal PpIX-enrichement.