2009•Unpublished venueRequires access

Sequence variants of E5 ORFs bovine papillomavirus detected in formalin-fixed and paraffin-embedded tissues obtained from equine sarcoids.

Anna Szczerba-Turek, Jan Siemionek, Wojciech Szweda, Agata Bancerz‐Kisiel, Andrzej Raś, T. Rotkiewicz

Abstract

The aim of the study was to identify the bovine papillomavirus (BPV) types associated with equine sarcoids observed in Polish horses, during 2001–2004. The samples of skin tumours were formalin-fixed and paraffin-embedded. Out of 14 tumours obtained from 10 horses, 11 were diagnosed as fibroblastic sarcoid, two as verrucose sarcoid, and one as epidermal cystoid. Using PCR, the presence of E5 open reading frames (ORFs) fragment BPV-1/BPV-2 were shown in all specimens obtained from archival tissues. Sequence variants of E5 ORFs confirm that European variants of BPV-1 occur in fibroblastic and verrucose equine sarcoids in Poland.

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Key concepts: ORFS, Bovine papillomavirus, Biology, Human papillomavirus, Open reading frame, Virology, Gene, Medicine