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2013•Journal of Herbs Spices & Medicinal PlantsRequires access

Phytochemical and Antibacterial Studies of Different Parts ofEuphorbia hirtaL.

Geeta Singh, Padma Kumar

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Abstract

Flavonoid extracts from Euphorbia hirta were screened for antibacterial activity by “Disc Diffusion Assay.” Minimum inhibitory concentration (MIC) of extracts was by broth microdilu-tion method. Extracts found sensitive against test pathogens were also evaluated for minimum bactericidal concentration (MBC) and total activity (TA). The highest inhibition zone (IZ) of 17 mm and AI 0.607 ± 0.090 was shown by bound flavonoids of leaves against Agrobacterium tumifacians. Bacillus subtilis was the most sensitive to flavonoids (free and bound) compared to the other bacteria. MIC of the extracts against Bacillus subtilis, Agrobacterium tumefaciens, and Klebsiella pneumoniae was recorded as 0.039 mg.mL−1.

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What this paper is about

Flavonoid extracts from Euphorbia hirta were screened for antibacterial activity by “Disc Diffusion Assay.” Minimum inhibitory concentration (MIC) of extracts was by broth microdilu-tion method. Extracts found sensitive against test pathogens were also evaluated for minimum bactericidal concentration (MBC) and total activity (TA). The highest inhibition zone (IZ) of 17 mm and AI 0.607 ± 0.090 was shown by bound flavonoids of leaves against Agrobacterium tumifacians. Bacillus subtilis was the most sensitive to flavonoids (free and bound) compared to the other bacteria. MIC of the extracts against Bacillus subtilis, Agrobacterium tumefaciens, and Klebsiella pneumoniae was recorded as 0.039 mg.mL−1.

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Available abstract

Flavonoid extracts from Euphorbia hirta were screened for antibacterial activity by “Disc Diffusion Assay.” Minimum inhibitory concentration (MIC) of extracts was by broth microdilu-tion method. Extracts found sensitive against test pathogens were also evaluated for minimum bactericidal concentration (MBC) and total activity (TA). The highest inhibition zone (IZ) of 17 mm and AI 0.607 ± 0.090 was shown by bound flavonoids of leaves against Agrobacterium tumifacians. Bacillus subtilis was the most sensitive to flavonoids (free and bound) compared to the other bacteria. MIC of the extracts against Bacillus subtilis, Agrobacterium tumefaciens, and Klebsiella pneumoniae was recorded as 0.039 mg.mL−1.

Key concepts: Euphorbia, Phytochemical, Bacillus subtilis, Minimum inhibitory concentration, Klebsiella pneumoniae, Minimum bactericidal concentration, Antibacterial activity, Flavonoid

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Phytochemical and Antibacterial Studies of Different Parts ofEuphorbia hirtaL. — Research Paper | ScholarLens