Isolation and Characterization of Siderophores from Azospirillum lipoferum D-2

Summary: Azospirillum Lipoferum strain D-2 produces the phenolate siderophores 2,3-dihydroxybenzoic acid, 3,5-dihydroxy benzoic acid and salicylic acid under iron-starved conditions. Lysine and leucine were identified as the amino acid conjugates of the siderophores. The concentration of siderophores in the culture supernatant was maximal after 20 h growth. Iron-binding proteins were present in membranes of cultures grown under iron starvation. Iron uptake was enhanced in the presence of each of the siderophores.