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Two-site assay of human α1-fetoprotein using125I-labelled monoclonal antibodies

Ian Weeks, Heather A. Kemp, J S Woodhead

Abstract

Monoclonal antibodies to human alpha 1-fetoprotein (AFP) have been compared with a conventionally produced antiserum using radioimmunoassay and two-site immunoradiometric techniques. A low-affinity antibody, which proved inadequate for use in a radioimmunoassay, gave a satisfactory dose-response curve in a rapid two-site assay. A higher-affinity antibody yielded a simple, rapid, and sensitive two-site assay suitable for routine measurement of serum AFP.

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Key concepts: Radioimmunoassay, Immunoradiometric assay, Monoclonal antibody, Antiserum, Antibody, Molecular biology, Chemistry, Alpha-fetoprotein