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Band broadening during dc and field inversion capillary electrophoresis of λ DNA in dilute hydroxyethylcellulose solutions

Jeffrey J. Schwinefus

Open publisher page 7 citations

Abstract

Capillary electrophoresis of lambda double-stranded (ds) DNA (48.5 kbp) in dilute hydroxyethylcellulose (HEC) solutions shows band spreading that cannot be explained by diffusion alone. Dispersion and asymmetry factors of lambda ds-DNA bands were measured as functions of capillary length, HEC concentration and field strength. Band spreading and asymmetry can be explained by a recently developed model in which the dominant contribution is assumed to be dispersion in DNA-HEC disentanglement times. Bandwidth reduction using square-wave field inversion was also investigated. It is proposed that correlation of DNA motion is the source of band narrowing during field inversion.

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What this paper is about

Capillary electrophoresis of lambda double-stranded (ds) DNA (48.5 kbp) in dilute hydroxyethylcellulose (HEC) solutions shows band spreading that cannot be explained by diffusion alone. Dispersion and asymmetry factors of lambda ds-DNA bands were measured as functions of capillary length, HEC concentration and field strength. Band spreading and asymmetry can be explained by a recently developed model in which the dominant contribution is assumed to be dispersion in DNA-HEC disentanglement times. Bandwidth reduction using square-wave field inversion was also investigated. It is proposed that correlation of DNA motion is the source of band narrowing during field inversion.

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Available abstract

Capillary electrophoresis of lambda double-stranded (ds) DNA (48.5 kbp) in dilute hydroxyethylcellulose (HEC) solutions shows band spreading that cannot be explained by diffusion alone. Dispersion and asymmetry factors of lambda ds-DNA bands were measured as functions of capillary length, HEC concentration and field strength. Band spreading and asymmetry can be explained by a recently developed model in which the dominant contribution is assumed to be dispersion in DNA-HEC disentanglement times. Bandwidth reduction using square-wave field inversion was also investigated. It is proposed that correlation of DNA motion is the source of band narrowing during field inversion.

Key concepts: Capillary electrophoresis, Lambda, Electrophoresis, Chemistry, Asymmetry, Analytical Chemistry (journal), Dispersion (optics), Capillary action

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