2014•Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Māzandarān/Journal of Mazandaran University of Medical SciencesOpen access
EVALUATION OF SURFACE MARKERS AND RELATED GENES OF THE HUMAN UMBILICAL CORD DERIVED WHARTON’S JELLY MESENCHYMAL STEM CELLS
Homa Mohseni Kouchesfahani, Saraee Farnoosh, Maleki Masoud, Nikougoftar Mahin, Khatami Seyedeh Mahsa, Mohsen Sagha
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Abstract
Background and purpose: Umbilical cord derived Wharton's jelly is an enrich ed and accessible source of stem cells with highly proliferative and differenti ation potential. This study aimed to evaluate the s urface markers and related genes of the stem cells isolated from t he human Wharton's jelly. Materials and methods: Explants of the human umbilical cord derived Wharton's jelly was dissected and cultured in Dulbecco's modified Eagle's medium (DMEM ) supplemented with 20% Fetal bovine serum (FBS). Then, those cells migrated from explant's boundary were r eplated and passaged in DMEM containing 10% FBS. Finally, by using flowcytometry and (Reverse-transcriptase polymerase chain reaction) RT-PCR techniques different surface markers and related genes were analyzed. Results: 5-7 days post-plating, the stem cells initiated th e migration from cultured explants and showed up to 80% densities on days 16-18. Light microscopy demonstrated two distinct cell populations including fibroblast- like and flat endothelial-like cells. Dual staining with flowcytometry also revealed that the cultured cells were found to be positive for CD44, CD73, CD90, CD105 and negative for CD34 and CD45. RT-PCR showed no changes in CD marker expression pattern during different passages . Conclusion: Human Wharton's jelly derived stem cells appear mesenchymal cell morphology and express related surface markers but no hematopoietic stem c ell markers.