2003•PubMedRequires access
Multiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.
Young Hee Lim, Kenji Hirose, Hidemasa Izumiya, Eiji Arakawa, Hideyuki Takahashi, Jun Terajima, Ken Ichiro Itoh, Kazumichi Tamura, Sung Il Kim, Haruo Watanabe
Open publisher page 89 citations
Abstract
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identification of S. enterica serovar Typhimurium.