Restriction Enzyme Analysis of Bacillus subtilis Bacteriophage 105 DNA
U. D. BUGAICHUK, Mary E. Deadman, Jeff Errington, Demetris Savva
Abstract
U. D. BUGAICHUK, Mary E. Deadman, Jeff Errington, Demetris Savva
Abstract
The recognition sites on phi 105 DNA for the restriction endonucleases EcoRI, Bg/II, SmaI, KpnI, SstI, SalI, XhoI, NcoI, PstI, HindIII, ClaI, EcoRV and MluI have been mapped. The sites for EcoRI are shown to be different from those published earlier. The DNA from phi 105 contains no recognition sites for the endonucleases BamHI and XbaI.
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The recognition sites on phi 105 DNA for the restriction endonucleases EcoRI, Bg/II, SmaI, KpnI, SstI, SalI, XhoI, NcoI, PstI, HindIII, ClaI, EcoRV and MluI have been mapped. The sites for EcoRI are shown to be different from those published earlier. The DNA from phi 105 contains no recognition sites for the endonucleases BamHI and XbaI.
Key concepts: Bacillus subtilis, Bacteriophage, Restriction enzyme, DNA, Enzyme, Biology, Microbiology, Genetics