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2010•Journal of Yeast and Fungal ResearchOpen access

Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes

Ganesh N. Sharma, Radha Raman Pandey

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Abstract

The mycelial growth rate, colony character and sporulation pattern of ten fungal isolates, grown on three different culture media viz., Potato Dextrose Agar (PDA), Czapek’s Dox + Yeast Extract Agar (CYA) and Lignocellulose Agar (LCA) were observed after seven days of incubation at 25±1οC. The colony diameter, culture characteristics (texture, surface and reverse colouration, zonation) and sporulation of selected test fungi were greatly influenced by the type of growth medium used. LCA exhibited comparatively higher mycelial growth in six test fungi, whereas all the ten isolates revealed heavy sporulation on this culture medium. Penicillium sp. and Acremonium kiliense exhibited maximum colony growth on PDA, whileChaetomium funicola and Fusarium oxysporum showed highest growth on CYA medium. These results will be useful for fungal taxonomic studies. Key words: Mycelial growth, colony character, sporulation, culture media.

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What this paper is about

The mycelial growth rate, colony character and sporulation pattern of ten fungal isolates, grown on three different culture media viz., Potato Dextrose Agar (PDA), Czapek’s Dox + Yeast Extract Agar (CYA) and Lignocellulose Agar (LCA) were observed after seven days of incubation at 25±1οC. The colony diameter, culture characteristics (texture, surface and reverse colouration, zonation) and sporulation of selected test fungi were greatly influenced by the type of growth medium used. LCA exhibited comparatively higher mycelial growth in six test fungi, whereas all the ten isolates revealed heavy sporulation on this culture medium. Penicillium sp. and Acremonium kiliense exhibited maximum colony growth on PDA, whileChaetomium funicola and Fusarium oxysporum showed highest growth on CYA medium. These results will be useful for fungal taxonomic studies. Key words: Mycelial growth, colony character, sporulation, culture media.

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Available abstract

The mycelial growth rate, colony character and sporulation pattern of ten fungal isolates, grown on three different culture media viz., Potato Dextrose Agar (PDA), Czapek’s Dox + Yeast Extract Agar (CYA) and Lignocellulose Agar (LCA) were observed after seven days of incubation at 25±1οC. The colony diameter, culture characteristics (texture, surface and reverse colouration, zonation) and sporulation of selected test fungi were greatly influenced by the type of growth medium used. LCA exhibited comparatively higher mycelial growth in six test fungi, whereas all the ten isolates revealed heavy sporulation on this culture medium. Penicillium sp. and Acremonium kiliense exhibited maximum colony growth on PDA, whileChaetomium funicola and Fusarium oxysporum showed highest growth on CYA medium. These results will be useful for fungal taxonomic studies. Key words: Mycelial growth, colony character, sporulation, culture media.

Key concepts: Spore, Mycelium, Agar, Biology, Potato dextrose agar, Acremonium, Penicillium, Fusarium oxysporum

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