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2013•The AnalystOpen access

Highly efficient ionization of phosphopeptides at low pH by desorption electrospray ionization mass spectrometry

Ning Pan, Pengyuan Liu, Weidong Cui, Bo Tang, Jing-Min Shi, Hao Chen

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Abstract

A fast and novel strategy for efficient ionization of phosphopeptides in mixtures is reported, in which the sample is acidified to low pH to suppress the deprotonation of phosphate groups and then followed by direct analysis using liquid sample desorption electrospray ionization mass spectrometry (DESI-MS).

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What this paper is about

A fast and novel strategy for efficient ionization of phosphopeptides in mixtures is reported, in which the sample is acidified to low pH to suppress the deprotonation of phosphate groups and then followed by direct analysis using liquid sample desorption electrospray ionization mass spectrometry (DESI-MS).

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OpenAlex reports 14 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A fast and novel strategy for efficient ionization of phosphopeptides in mixtures is reported, in which the sample is acidified to low pH to suppress the deprotonation of phosphate groups and then followed by direct analysis using liquid sample desorption electrospray ionization mass spectrometry (DESI-MS).

Key concepts: Chemistry, Desorption electrospray ionization, Extractive electrospray ionization, Mass spectrometry, Electrospray ionization, Ionization, Direct electron ionization liquid chromatography–mass spectrometry interface, Desorption

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