Molecular detection of endophytic, myrothecium spp. by its-Sequencing Technique

Endophytic fungi are micro organisms with immense production secondary metabolites having biological activity features, \nwhich exist withinplantswithout anyhazard for their living \nTen isolates of endophytic Myrothecium ver rucaria \nspecies, recovered from bark and twigs of Garcinia spp. in order to detection and identification of this fungus plant, \nand to analyze their genome fingerprints with help of ITS \n- sequencing. ITS region is one of the most famous nuclear DNA sequences for genetic inference at the generic and genus levels in fungus. Hence,the target regions of rDNA, ITS 1, ITS 2 regions and 5.8S gene were amplified using primers ITS 1 and ITS 4.In this research,the phylogenetic evolutionary relationship of the desired endophyte were evaluated from the sequences of the ITS region.The amplification and subsequent sequencing of highly conserved ITS region showed genetic similarity among 10 isolates of \nMyrothecium spp (M), and based on the polymorphisms of the ITS region, the 10 isolates gave a length of ~550 bpamplicon