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2011•MycobiologyOpen access

Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus,Gliocladiumsp., fromTaxus baccata

Dasari Sreekanth, G.K. Sushim, Asad Syed, Bashir M. Khan, Absar Ahmad

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Abstract

The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

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The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

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Available abstract

The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

Key concepts: Taxus, Biology, Plant use of endophytic fungi in defense, Internal transcribed spacer, Botany, Ascomycota, Fungus, Ribosomal RNA

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Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus,Gliocladiumsp., fromTaxus baccata — Research Paper | ScholarLens