2005•Antimicrobial Agents and ChemotherapyOpen access
Anucleate Cell Blue Assay: a Useful Tool for Identifying Novel Type II Topoisomerase Inhibitors
Yoshihiro Oyamada, Hideaki Ito, Mika Fujimoto-Nakamura, Akihiko Tanitame, Noritaka Iwai, Kazuo Nagai, Jun‐ichi Yamagishi, Masaaki Wachi
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Abstract
About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.