2005•Applied and Environmental MicrobiologyOpen access

Symbiotic Hydrogenase Activity in Bradyrhizobium sp. ( Vigna ) Increases Nitrogen Content in Vigna unguiculata Plants

Cecilia Baginsky, Belén Brito, Juan Imperial, Tomás Ruiz‐Argüeso, José Manuel Palacios

Abstract

Bradyrhizobium sp. (Lupinus) and Bradyrhizobium sp. (Vigna) mutants in which hydrogenase (hup) activity was affected were constructed and analyzed. Vigna unguiculata plants inoculated with the Bradyrhizobium sp. (Vigna) hup mutant showed reduced nitrogenase activity and also a significant decrease in nitrogen content, suggesting a relevant contribution of hydrogenase activity to plant yield.

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Key concepts: Vigna, Bradyrhizobium, Nitrogenase, Bradyrhizobium japonicum, Nitrogen fixation, Biology, Hydrogenase, Inoculation