2006•CytopathologyOpen access
Performance of the DNA‐Citoliq liquid‐based cytology system compared with conventional smears
Venâncio Avancini Ferreira Alves, Adauto Castelo, A. L. Filho, Maria Regina Vianna, Eliane Taromaru, Gislene Mitsue Namiyama, Attila T. Lörincz, Gerson Botacini das Dôres, DNA‐Citoliq Working Group, São Paulo, Brazil
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Abstract
OBJECTIVE: To evaluate the performance of a new, manual, simplified liquid-based system, DNA-Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories. METHODS: A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions. RESULTS: Of 3206 pairs of slides considered valid for comparison, there were 3008 in full agreement (93.8%), 112 (3.5%) with one diagnostic category discrepancies, and 86 (2.7%) discordant cases. Among the 288 borderline+ by either method, DNA-Citoliq detected abnormalities in 243 (84.4%), and conventional smears (CS) detected abnormalities in 178 (61.8%) (McNemar test, P < 0.000), a 36.5% increased detection of borderline+ cases. CONCLUSIONS: For mild dyskaryosis, DNA-Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).