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2010•Journal of Clinical MicrobiologyOpen access

Novel Multiplex PCR Using Dual-Priming Oligonucleotides for Detection and Discrimination of the Mycobacterium tuberculosis Complex and M. bovis BCG

Hye Ryun Lee, So Yeon Kim, Ho Eun Chang, Sang Hoon Song, Hye Seung Lee, Kyoung Un Park, Junghan Song, Eui-Chong Kim

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Abstract

We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.

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We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.

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Available abstract

We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.

Key concepts: Mycobacterium bovis, Mycobacterium tuberculosis complex, Mycobacterium tuberculosis, Tuberculosis, Biology, Multiplex polymerase chain reaction, Multiplex, Oligonucleotide

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