Direct Enzymatic Assay for Alcohol Oxidase, Alcohol Dehydrogenase, and Formaldehyde Dehydrogenase in Colonies of Hansenula polymorpha
Lothar Eggeling, Hermann Sahm
Abstract
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Lothar Eggeling, Hermann Sahm
Abstract
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A procedure is described for the qualitative direct identification of alcohol oxidase, alcohol dehydrogenase, and formaldehyde dehydrogenase in yeast colonies. The method has been applied successfully to isolate mutants of Hansenula polymorpha with altered glucose repression of alcohol oxidase.
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A procedure is described for the qualitative direct identification of alcohol oxidase, alcohol dehydrogenase, and formaldehyde dehydrogenase in yeast colonies. The method has been applied successfully to isolate mutants of Hansenula polymorpha with altered glucose repression of alcohol oxidase.
Key concepts: Alcohol oxidase, Alcohol dehydrogenase, Formaldehyde dehydrogenase, Alcohol oxidoreductase, Biochemistry, Enzyme, Yeast, Dehydrogenase