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1966•Journal of Experimental BotanyRequires access

Studies on Isolated Protoplasts and Vacuoles: I. GENERAL PROPERTIES

D. W. Gregory, E. C. Cocking

Open publisher page 10 citations

Abstract

Protoplasts and vacuoles have been isolated in large quantities from the parenchymatous placental tissue of immature tomato fruit. Their mechanical stability and osmotic stability have been investigated. A small proportion of initially discrete but adjacent vacuoles were seen to coalesce; the possible significance of this phenomenon in the growth of plant cells is discussed. The overall chemical composition of isolated protoplasts has been determined, and their lipids have been analysed by thin-layer chromatography.

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What this paper is about

Protoplasts and vacuoles have been isolated in large quantities from the parenchymatous placental tissue of immature tomato fruit. Their mechanical stability and osmotic stability have been investigated. A small proportion of initially discrete but adjacent vacuoles were seen to coalesce; the possible significance of this phenomenon in the growth of plant cells is discussed. The overall chemical composition of isolated protoplasts has been determined, and their lipids have been analysed by thin-layer chromatography.

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Available abstract

Protoplasts and vacuoles have been isolated in large quantities from the parenchymatous placental tissue of immature tomato fruit. Their mechanical stability and osmotic stability have been investigated. A small proportion of initially discrete but adjacent vacuoles were seen to coalesce; the possible significance of this phenomenon in the growth of plant cells is discussed. The overall chemical composition of isolated protoplasts has been determined, and their lipids have been analysed by thin-layer chromatography.

Key concepts: Protoplast, Vacuole, Botany, Library science, Biology, Computer science, Biochemistry, Cytoplasm

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