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PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST EARLY POLISH STRAINS OF INFECTIOUS BURSAL DISEASE VIRUS

Zenon Minta, Katarzyna Domańska-Blicharz, B. Bartnicka, Krzysztof Śmietanka

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Abstract

Monoclonal antibodies (Mabs) were produced against the early Polish strains of infectious bursal disease virus (IBDV) isolated from subclinical cases of Gumboro disease between 1978 and 1980. Splenocytes from immunized mice were fused to myeloma cells and hybridomas producing antibodies against homologous and different pathotype of IBDVs were selected by indirect ELISA, immunoperoxidase, ELISA-VP2, virus neutralization (VN), Western blotting and additivity ELISA methods. The group of monoclonal antibodies which could be used for rapid detection of IBDV and identification of field isolates was obtained. Mab IIIE6 could be used to detect F52/70 IBDV and Mab VD2 to detect “early Polish-like” viruses in indirect ELISA. Mabs IID5 and IIIC6 neutralized only one vaccinal strain (Lukert) in VN assay.

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What this paper is about

Monoclonal antibodies (Mabs) were produced against the early Polish strains of infectious bursal disease virus (IBDV) isolated from subclinical cases of Gumboro disease between 1978 and 1980. Splenocytes from immunized mice were fused to myeloma cells and hybridomas producing antibodies against homologous and different pathotype of IBDVs were selected by indirect ELISA, immunoperoxidase, ELISA-VP2, virus neutralization (VN), Western blotting and additivity ELISA methods. The group of monoclonal antibodies which could be used for rapid detection of IBDV and identification of field isolates was obtained. Mab IIIE6 could be used to detect F52/70 IBDV and Mab VD2 to detect “early Polish-like” viruses in indirect ELISA. Mabs IID5 and IIIC6 neutralized only one vaccinal strain (Lukert) in VN assay.

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Available abstract

Monoclonal antibodies (Mabs) were produced against the early Polish strains of infectious bursal disease virus (IBDV) isolated from subclinical cases of Gumboro disease between 1978 and 1980. Splenocytes from immunized mice were fused to myeloma cells and hybridomas producing antibodies against homologous and different pathotype of IBDVs were selected by indirect ELISA, immunoperoxidase, ELISA-VP2, virus neutralization (VN), Western blotting and additivity ELISA methods. The group of monoclonal antibodies which could be used for rapid detection of IBDV and identification of field isolates was obtained. Mab IIIE6 could be used to detect F52/70 IBDV and Mab VD2 to detect “early Polish-like” viruses in indirect ELISA. Mabs IID5 and IIIC6 neutralized only one vaccinal strain (Lukert) in VN assay.

Key concepts: Infectious bursal disease, Monoclonal antibody, Virology, Virus, Immunoperoxidase, Antibody, Biology, Molecular biology

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PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST EARLY POLISH STRAINS OF INFECTIOUS BURSAL DISEASE VIRUS — Research Paper | ScholarLens