2009•Antimicrobial Agents and ChemotherapyOpen access

aac(6′)-Ib-cr Genotyping by Simultaneous High-Resolution Melting Analyses of an Unlabeled Probe and Full-Length Amplicon

Jan M. Bell, John D. Turnidge, Patiyan Andersson

Abstract

We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.

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Available abstract

Key concepts: Genotyping, Amplicon, High Resolution Melt, Resolution (logic), Molecular biology, Biology, Polymerase chain reaction, Genetics