Quantification of Pharmacologically Active Markers Gallic Acid, Quercetin and Lupeol from Acacia Leucophloea Wild Flowers by HPTLC Method

Objective: TLC densitometric method for quantification of gallic acid, quercetin and lupeol using HPTLC is developed. This is the first report of quantification of these three bioactive compounds viz. Gallic acid, quercetin and lupeol using HPTLC from this plant. Methodology: Quantification of gallic acid and quercetin was carried out from the methanolic extract using the solvent system of Toluene:Ethyl acetate:Formic acid (6:4:0.8 v/v/v). Lupeol was quantified from chloroform extract using the solvent system of Toluene:Ethyl acetate (7:3 v/v). Results: The Rf values of gallic acid, quercetin and lupeol are 0.22, 0.37 and 0.70 respectively. The linearity ranges for gallic acid (100 to 600 ng), quercetin (2000 to 7000 ng) and lupeol (100 to 1200 ng) with correlation coefficients (r-values) of 0.99968, 0.99708, and 0.99971 respectively. The amount of gallic acid, quercetin and lupeol was 124.31, 580.4, 24.89 μg/ml respectively. Conclusion: Quantification of gallic acid, quercetin and lupeol showed good resolution and separation from other constituents of extract. Its main advantages are its simplicity, accuracy, and selectivity. This method can also be used for the estimation of these compounds in other herbal preparations and may be useful for standardization purposes.