1995•Journal of BacteriologyOpen access

Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis

Alphons J.A.M. van Asten, Kornelisje A. Zwaagstra, Marc F.D. Baay, Johannes G. Kusters, J H Huis in't Veld, Bernard A.M. van der Zeijst

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Abstract

Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.

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Key concepts: Flagellin, Salmonella enteritidis, Biology, Epitope, Microbiology, Serotype, Salmonella, Gene