2011Journal of Clinical MicrobiologyOpen access

High-Resolution Melting Analysis for Identification of the Cryptococcus neoformans-Cryptococcus gattii Complex

Sara Gago, Óscar Zaragoza, Isabel Cuesta, Juan L. Rodrı́guez-Tudela, Manuel Cuenca‐Estrella, María José Buitrago

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Abstract

We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

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What this paper is about

We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

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Available abstract

We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

Key concepts: Cryptococcus neoformans, Cryptococcus gattii, Cryptococcosis, Cryptococcus, Biology, High Resolution Melt, Microbiology, Genetics

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