2010•Cell Biology InternationalRequires access
Effect of angiotensin(1-7) on the expression of E-selectin induced by angiotensinII and its mechanism in vascular endothelial cell
Xiaohui Shen, Zhibin Wen, Na Li, Qingmei Cheng, Xiaofan He, Shilin He
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Abstract
Objective. To evaluate the effects of angiotensin II on the expression of tissue factor (TF) in the cell line ECV304 and the effects of angiotensin (1-7) on induced TF angiotensin II expression. Methods. ECV304 cells were cultured in RPMI1640 medium. The procoagulant activity (PCA) was determined with one-stage clotting assay and TF mRNA examined by semi-quantitative RT-PCR. Immunohistochemical analysis was performed to evaluate the activation of NF-κB. Results. Compared with the control, angiotensin II (10 –10 – 10 –6 mol/L) significantly increased PCA of ECV304 cells and TF mRNA expression in a dose-dependent manner (P < 0.01, r = 0.9631) and the peak concentration was 10 –6 mol/L. Angiotensin (1-7) (10 –10 – 10 –7 mol/L) alone could not change the PCA, but pretreated the ECV304 cells with angiotensin (1-7), which