Induced spawning and larval rearing of grouper (Epinephelus salmoides Maxwell)

Broodfish of grouper (Epinephelus salmoides ) were induced to spawn by hormonal induction. The hormones used for this experiment were HCG + pituitary gland (PG) and LRH-a. The results showed that at dosages of 500 IU HCG + 3 mg of PG per kg of fish for first injection and 1,000 IU HCG + 3 mg of PG per kg of fish at the final injection at an interval of 24 hours, the treated fish spawned naturally in a spawning tank 12 hours after the final injection. At lower dosages of 500 IU HCG + 3 mg PG at 12-hour intervals or 500 IU HCG + 3 mg PG at 24-hour intervals or using 10 mg LRH-a at 12-hour intervals, the eggs can be artificially fertilized only by stripping. The larval rearing experiment was conducted in a 250-l fiberglass tank at the stocking density of 2,500 larvae per tank. Feeds used in this experiment were: (a) Isochrysis + sea urchin eggs; (b) Isochrysis + Brachionus ; and (c) Tetraselmis + Brachionus . The results showed that newly hatched larvae fed with Isochrysis mixed with sea urchin eggs and then Brachionus from day 10 had the best survival rate (9% at day 30).