Genotyping of Some Local Isolates of Genus Salmonella Using RAPD-PCR
Nadine A. El-Sebay, Hassan M. Gebreel, A. A. Samy
Abstract
Nadine A. El-Sebay, Hassan M. Gebreel, A. A. Samy
Abstract
Abstract: Fourteen selected isolates of Salmonella were obtained from Kalubia and Giza governorates were isolated from different poultry spp. (chickens, chicken eggs, ducks, duck eggs and turkeys). These isolates were serotyped as: Salmonella rubislaw, Salmonella poona, Salmonella typhimurium, Salmonella virginia, Salmonella enteritidis, Salmonella montevideo and Salmonella sandiago. Random amplified polymorphic DNA (RAPD-PCR) analysis was carried out on all isolates by using five random primers. Primers (2), (3) and (5) could be used as serotype-specific primers in differentiation between Salmonella isolates. While primers (1) and (4) couldn’t be used as serotype-specific primers in differentiation between Salmonella isolates.
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Abstract: Fourteen selected isolates of Salmonella were obtained from Kalubia and Giza governorates were isolated from different poultry spp. (chickens, chicken eggs, ducks, duck eggs and turkeys). These isolates were serotyped as: Salmonella rubislaw, Salmonella poona, Salmonella typhimurium, Salmonella virginia, Salmonella enteritidis, Salmonella montevideo and Salmonella sandiago. Random amplified polymorphic DNA (RAPD-PCR) analysis was carried out on all isolates by using five random primers. Primers (2), (3) and (5) could be used as serotype-specific primers in differentiation between Salmonella isolates. While primers (1) and (4) couldn’t be used as serotype-specific primers in differentiation between Salmonella isolates.
Key concepts: Salmonella, Serotype, RAPD, Biology, Genotyping, Salmonella enteritidis, Microbiology, Veterinary medicine