2007•Journal of Clinical MicrobiologyOpen access

Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci

Kunyan Zhang, Jo‐Ann McClure, Sameer Elsayed, Thomas Louie, John Conly

Abstract

We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.

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Key concepts: Staphylococcus aureus, Leukocidin, Panton–Valentine leukocidin, Microbiology, Multiplex, Biology, Multiplex polymerase chain reaction, Staphylococcal infections