2002•Journal of Experimental ZoologyRequires access

Microsatellite DNA library for Caiman latirostris

Rodrigo Barban Zucoloto, Luciano M. Verdade, Luiz Lehmann Coutinho

Abstract

New genetic markers were characterized for the broad-snouted caiman (Caiman latirostris) by constructing libraries enriched for microsatellite DNA. Construction and characterization of these libraries are described in the present study. One microsatellite marker was developed from a (ACC-TGG)(n)enriched microsatellite DNA library, and 12 microsatellite markers were developed from a (AC-TG)(n)enriched microsatellite DNA library. These markers were tested in wild-caught animals, and these tests resulted in ten new polymorphic microsatellites for C. latirostris.

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Key concepts: Microsatellite, Biology, Zoology, Genetics, Gene, Allele