2001•Unpublished venueRequires access

Restriction Fragment Length Polymorphism Analysis (RFLP) of Some Streptomyces Strains from Soil

Ekrem Atalan

Abstract

Abstract: To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis. A dendrogram were constructed using the unweighed pair group method using arithmetic averages algorithm (UPGMA) after analysis restriction patterns. Five RFLP groups were obtained and one test strain was left as a single member group. RFLP profile indicated that unknown strains could be identified with data based on the interspacer region of 16S-23S DNA rapid and quickly.

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Available abstract

Key concepts: HaeIII, Restriction fragment length polymorphism, Biology, Restriction enzyme, Terminal restriction fragment length polymorphism, UPGMA, Genetics, Dendrogram