2015•HemoglobinOpen access

Determination of Human Hemoglobin Derivatives

Atef Attia, Fatma A. Ibrahim, Noha A. Abd El-Latif, Samir W. Aziz, Sherif A. Abdelmottaleb Moussa, Mohsen Saleh Elalfy

Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.

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Key concepts: Methemoglobin, Carboxyhemoglobin, Hemoglobin, Chemistry, Chromatography, Biochemistry, Carbon monoxide, Catalysis