2012•Journal of Clinical MicrobiologyOpen access

Rapid Determination of Lymphogranuloma Venereum Serovars of Chlamydia trachomatis by Quantitative High-Resolution Melt Analysis (HRMA)

Jimmy Twin, Matthew P. Stevens, Suzanne M. Garland, Angelo Zaia, Sepehr N. Tabrizi

Abstract

A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars of Chlamydia trachomatis (L1 to L3) from other C. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.

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Key concepts: Lymphogranuloma venereum, Chlamydia trachomatis, Serotype, Chlamydiaceae, Virology, Chlamydiales, Sexually transmitted disease, Biology