2010•Applied and Environmental MicrobiologyOpen access
Detection of Mycobacterium avium subsp. paratuberculosis in Drinking Water and Biofilms by Quantitative PCR
Amy Beumer, Dawn King, Maura J. Donohue, Jatin H. Mistry, Terry C. Covert, Stacy Pfaller
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Abstract
It has been suggested that Mycobacterium avium subspecies paratuberculosis has a role in Crohn's disease. The organism may be acquired but is difficult to culture from the environment. We describe a quantitative PCR (qPCR) method to detect M. avium subsp. paratuberculosis in drinking water and the results of its application to drinking water and faucet biofilm samples collected in the United States.