Plant regeneration after head cabbage (Brassica oleraceae var. capitata) seed treatment by γ-rays 60Co.
Stanislava Grozeva, Величка Родева, G. Antonova
Abstract
Stanislava Grozeva, Величка Родева, G. Antonova
Abstract
The aim of present investigation is characterization of changed head cabbage forms obtained after seed irradiation with 800 Gy γ-rays 60 Со and in vitro regeneration. There were registered the highest frequency of changes in pollen fertility (83,3% of plants) followed by plant height (66,7% of plants) of M1 generation in generative stage of development. The changes in some characteristics of flower and leaf morphology were observed also. We suppose that except applied physical mutagen in vitro cultivation of treated explants also effect the establishment of genetic diversity in this case. The obtained sterile and low pollen fertility head cabbage lines are of breeding interest for F1 hybrids creation.
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The aim of present investigation is characterization of changed head cabbage forms obtained after seed irradiation with 800 Gy γ-rays 60 Со and in vitro regeneration. There were registered the highest frequency of changes in pollen fertility (83,3% of plants) followed by plant height (66,7% of plants) of M1 generation in generative stage of development. The changes in some characteristics of flower and leaf morphology were observed also. We suppose that except applied physical mutagen in vitro cultivation of treated explants also effect the establishment of genetic diversity in this case. The obtained sterile and low pollen fertility head cabbage lines are of breeding interest for F1 hybrids creation.
Key concepts: Biology, Pollen, Brassica, Hybrid, Horticulture, Explant culture, Capitata, Botany